5A spare fuses, 2 cleaning needles, tubing sets for all bottles, cleaning tool for needles. Please refer to the Obsolete Microplate Reader Models for more information. VMax . 039. . 24-, 96-, 384-well plates and microwell strips; Manifold Channels: 96-well washing: 8-well (1x8) manifold, 2x8-well manifold, 12-well (1x12) manifold, 8-well short tube (1x8) manifold. Loading Buffer was also added to a set of cell-free wells for background controls. 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. 0025 OD from 0. x. UT-6550 Microplate Reader is a reliable and robust instrument for a wide. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. Some will only support one well density (e. • 0. the Gemini XPS reader. Two days before the experiments, cells are seeded into the clear-bottom 96-well plates (approximately 10,000 cells per well for cancer cell lines and 30,000–40,000 cells per well for fibroblasts and NPC; see Note 5) and cultured in the media suited for users’ cell lines. 0% ± 0. Four wash/rinse bottles are included and configurable for both 96- and 384. Some instruments can handle only 96-well plates and lower, whereas others can read up to the ultrahigh throughput densities of 1,536-well plates. Place the microplate in the drawer. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. It offers extremely low absorbance background, excellent. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. Note: if all wells of a 384-well plate are to be read, the kinetic interval may need to be. Top and bottom reads are. These robust readers have been placed in labs from Antarctica to the. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. 0 OD. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Plate SpectraMaxiD3,iD5,i3x,i3,M2,M2e,M3,M4,M5,M5e,Plus. HeLa. Molecular Devices: EMax Plus: SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise:. White 96-well platePlate formats. • 96-well yellow plate 0200-6182 Pipettor head kit, 16-channel (384) for FlexStation 3 Reader • 16-channel pipettorElisa Plate Readers and Microplate Readers at Biocompare. 2 with 1000 cells. • Gently wash cells once with 200 μl (for 96 well plate) or 400 μl (for a 24 well plate) warm (37°C) 1X DPBS (containing Mg and CaCl 2). 1 – 2. The eight-channel system delivers high precision and speed when reading 96-well microplates for single endpoint measurements and kinetic assay data gathered over time. 0A-Sunnyvale, CA-Made in USAWe offer a range of multiwell plates including 384-well, 96-well and 24-well plates with a selection of membrane types:. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. High tolerance for contaminants. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. The luminescence was measured in a SpectraMax. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell concentration left to right starting at Col. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. explore more. Our washers provide speed and configurability options—from single-tube dispensing to washing full 96- and 384-well plates. They are also sealable using different types of adhesive mats foils or films. Determine concentration using the Beer-Lambert LawPage 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. #E010) Pyroplate 96-well microplate (ACC cat. Now select the area of the plate to read. com Molecular Devices (Germany) GmbH ismarcring ierach an der iss erman ©2019 Molecular Devices, LLC. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. QuickSync. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. With theMolecular DevicesWe designed and fabricated different sized microfluidic covers, matched to the desired experimental conditions: Fig. All readings were taken in clear, polystyrene 96-shallow-well, flat-bottom plates. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. 25, 3. Supersedes 1. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the assay. Single or dual wavelength read, kinetic readings, automix, endpoint, and blank pattern, curve fits included with PC Software. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. An example of dual-luciferase assay results. Infinite possibilities. For biochemical assays, the washer removes unbound material and unreacted reagents. We would like to show you a description here but the site won’t allow us. Three low volume 24-well bottom slides. It features a modular design that allows users to add or change detection cartridges according to their needs. UV cuvettes are ideally suited for measurements at 260 nm, 280 nm and in the visible range. Compare this item. x. The drawing shows a standard microplate of 15 mm height covered with a lid. It has a 340-750nm Spectral Range. When a. In the latter case, 8 wells in each column are perfused in series and the 12 columns are. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 or 384 wells) and the "Select Specific" menu to choose the specific plate type. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for. Accurate, efficient nucleic acid quantitation is critical for molecular biology labs that work with precious, rare, or difficult-to-process samples and applications requiring precise measurement. 96-, 384-, and 1536-well microplates. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. Temperature variation ± 0. The clear bottom plates offer the advantage of being able to examine the cells by microscopy during the course of the. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. SoftMax Pro Software minimizes setup time for detection and automates standard curve fitting and sample quantitation. 4: Berthold Technologies: TriStar LB 941: Multimodal plate reader: Yes:. When a. UV absorbance detection. Growth medium was then replaced with fatty acid uptake solution. This SpectraMax Plus comes from a long history of reliable tools built by Molecular Devices. Both the P and M injectors were set to deliver 100 µL (96-well plate) or 25 µL (384-well plate), with a 2-second delay and 10-second integration time. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 5 second read per plate. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. External Dimensions without lid (mm). Description. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Primary considerations when choosing a microplate reader are throughput and flexibility. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. Products : ClonePix 2 System, QPix 450/460 Systems, QPix 420 System, CloneSelect Imager. Three kits covering a wide range of dsDNA concentrations. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell. Measurements for every well are automatically tracked over time. Corning. Answer. #iD5-STD). This reader can also be used as a standard. 2. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. 96-well) while others have flexibility to. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Two different agonists, acetylcholine and carbachol, were tested. 45 µm PVDF. x. Expand Microplates and select the newly created plate. Supplier: Molecular Devices. Sensitive luminometer with programmable injector options for 96- and 384-well microplates. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl U-bottom: 30-40 µl flat-bottom: 50-60 µl 384-well plates: approximately 20 µl. For a 96-well plate it takes a total of 27 seconds to switch between wells and then you have to add the actual injection time based on the injection rate entered by the user. • High antibody-binding surface improves the overall performance in. The 8-channel read head allows for fast read times which means a 96-well plate can be read in as little as five seconds. 9000-0756. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). The Best Microplate Readers According to our Product Reviewers (1) Write a Review. These plates are compatible with standard fluorescence microplate readers and are offered in a. #SpectraMax L Config) Methods. The CellTiter-Glo assay was used to measure ATP. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Molecular Devices LLC Applications: Inquire; Plate Formats: 96-, 384- well. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. We would like to show you a description here but the site won’t allow us. 96- and 384-well. Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Robust shaking and consistent temperature control optimize bacteria and yeast cell growth. Published Dec 16, 2020 | Updated Jun 22, 2023. Other. Back plate and…. one 96-well sample plate can be controlled from 15–40°C,. High sensitivity and speed are required for these plate formats if you wish to examine quick kinetic measurements such as calcium fluxes or applications with small signal. ZERO BIAS - scores, article reviews, protocol conditions and moreThe VersaMax Absorbance Microplate Reader measures the optical density (OD) of samples at selected wavelengths in a number of reading modes. Microplates, cuvettes (via adapter) Detectors. Multimodal plate reader: Yes: 25 mm: Yes—Max. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. The CellTiter-Glo assay was used to measure ATP. Silica excitation fiber, glass emission fiber. Materials. x. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 000 – 4. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. Step 2: Add sample to well – antigen within the sample binds to the capture antibody. C. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. No accessories are needed and SoftMax® Pro Software. Reader Documentation Version No. Molecular Devices L-Max II Luminescence Microplate Reader. Sample by eye within . Products : SpectraMax L reader How long does it take to dispense into a 96 well plate with SpectraMax L reader injection? For a 96-well plate it takes a total of 27. Dynamic range > 6 decades . 0 Optical system Filters Power requirementAfter reading the ELISA plate on the EMax® Plus Microplate Reader, the R2 value was 0. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. maximum-illumination-channels. 1 x plates; Instrument dimensions. Temperature control: 4°C above ambient to 45°C. Page 20: Plate Adapter SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. Wide range of applications The VersaMax reader covers a wide range of applications: Step 1: Turn on your microplate reader. 4 (Molecular Devices cat. 000 ABS • Resolution: 0. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…AquaMax® Microplate Washers are configurable with user interchangeable heads for both 96- and 384-well microplate washing applications, including plate or cell washing. 5 OD at 492 nm Computer interface USB 2. For the second test, the validation kit contained 36 known samples, including 33 DNA samples and 3 no-template controls (NTC). #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. SpectraMax® Microplate Reader Suite of Products Knowledge Base. Software Validation. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. The BCA assay plate was read at 562 nm, the original wavelength setting in the protocol, while for the BCA Rapid. Molecular Devices, LLC Plate Formats: 96 or 384 Well Plates; Manifold Channels: Inquire; Dispensing Liquids: Inquire; Citations: Get Quote. All trademarks used herein are the property of. variety of. The procedures in this protocol support the 96-well reaction plate. #ABS PLUS) Methods. Two high-energy xenon flash lamps: Reading capabilities. Includes PC and software. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. #3603) SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. robotics-automation-compatible. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of the human AT1R receptor. SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701). This instrument is ideal for measuring dual luciferase assays in both 96- and 384-well plates, and SoftMax Pro® software simplifies data collection and analysis with preconfigured protocols. Select up to 3 products. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. A = 2 - log 10 (%T). An industry-renowned instrument for monitoring GPCRs and ion channels. 96-well-plate-scan-time-2-colors-1-site-well. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. Figure 1-2 Match chamfered corners of slide and adapter 2. x. 5 pM Luminescence performance Wavelength range 300–850 nm Wavelength selection Choice of simultaneous detection of all wavelengths or selection in 1. 1. x. The. Click Add to create the plate. Download a PDF version of the SpectraTest validation package User Guide and the ABS2 SpectraTest validation protocols. Temperature control range from Ambient +4º to 50ºC. Place the microplate in the drawer. The color touch screen provides a visual user interface, making programming fast and intuitive. Optional 12-way manifold) 5032336. No tools required to assemble. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. 046 to 0. 315 can't find zero order. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. a white 96-well half-area microplate (Corning cat. Plate Background OD, which was used for subsequent tests, was measured as follows. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. 2. Published Dec 16, 2020 | Updated Dec 16, 2020. Features of Clear 96-Well Microplates: • Clear plates for colorimetric ELISA and other assays. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. 312 gain calibration failed. Published Dec 16, 2020 | Updated Dec 06, 2021. µl) was added to each well and incubated at 37°C for 1 h. Washers configured for both 96- and 384-well plates. Gemini EM Reader scans. Opaque-walled 96- or 384-well tissue culture plates are available as white or black with solid bottoms and white or black with clear bottoms. x. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. • Remove media from each well using a blue pipette tip being careful as not to remove cells. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 76358-624. Molecular Devices SpectraMax Plus Microplate Reader Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader Sale Price: $6,495. Molecular Devices delivers a robust screening platform to address real-time kinetic cell-based assays with the FLIPR Tetra® System. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. The screen on the left shows the wells selected for Method MI AF488-10 and Method MI AF488-5 for image acquisition from rows A-D of a 96-well plate at. Polypropylene deep well plates, round bottom. 005 OD Normal and Rapid modes, 96- and 384-well plates;. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. Controlled by the computer. 00 . Linear, Orbital Slow, and Orbital Fast. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Plates were read in the SpectraMax M5 microplate reader at 485ex/515em with bottom read. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. What is the dead volume in 96 and 384 well compound plates? Dead volume is 5 micro liters. This reader's dual monochromators for. Quantitative, whole well cell confluence for every well. ₹ 1,80,000 Get Latest Price. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. A custom 96-well plastic (polyethylene terephthalate/PET) plate is attached via epoxy to the mounted CMOS ICs, designed with a standard form factor to be compatible with high-throughput. 020 fmol 0. Never order or replace filters to optimize an assay again!6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. These instruments deliver high precision and speed when reading 96 Well Microplates for single endpoint measurements and kinetic assay data gathered over time. SpectraMax i3x Multi-Mode Microplate Reader. Luminometers and fluorimeters allow for readout of luminescence (luciferase readouts) or fluorescence from an entire well of a micro-well plate such as a 96- or 384-well plate. Source plate capacity; 1 x plates. and the A1 corner of the plate adapter (Figure 1-2). The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. sdale innersh Triangle Woingham ershire RG41 T United Kingdom 11 umolde. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well…. In addition, users can define individual reagents and concentrations to be delivered to each well. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. to each well. 96- or 384-well microplate compatibility. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andthe iD5 reader’s filter system. To validate the assays’ ability to detect a wide range of viable cell numbers, a series of cell densities was set up. Well scanning With well scanning, multiple points within each well can be read, providing a high. VIS absorbance detection. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. Molecular Spectroscopy Consumables & Accessories Thermal Analysis. as well as all other Molecular Devices' microplate readers. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. Published Dec 16,. Users can choose to process all plates within the magazine or select a specific number. Figure 1. 4D-Nucleofector 96-well Shuttle: RATE CONTROLLED. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Molecular Devices (UK) Ltd. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. EMax. $3,450. Products Clone Screening Accessories and Consumables Deep-well Plates Deep-well Plates Deep well blocks available in 24, 96 and 384-well formats Product Options. The device was able to achieve 3σ limits of detection of ca. 303 unable to cal dark current. Step 3: Click “Settings” and a screen will appear (Figure 1). Thermo Scientific Pierce Streptavidin Coated Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. View Product. Fluorescence Microplate Reader Comparison Chart. 5% at 1. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Up to 96 well plates, there is often a good signal that can easily be detected, and 96 wells are measured comparatively quickly. Compare this item. 6-384 (96- and 384-well with pipetting) 96-well microplates. 340 PC384. 984. 96 wells 384 wells 1 pM 1 pM Optimized bottom sensitivity (fluorescein) 96 wells 384 wells 2 pM 2. To assess the sensitivity of each microplate reader, a plate was set up with 32 wells containing PBS only. The patented quench technology is offered to drug discovery and life science researchers exclusively by Molecular Devices, through the purchase of FLIPR Assay Kits. Published Dec 16, 2020 | Updated Dec 16, 2020. Kinetic assays were read every 20-28 seconds for one hour,The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Click Create. Versa Max. What are the dimensions of the plate? Code. Microplate readers, SpectraMax®, with SpectraDrop. 5 ng per well. Self-righting base, up to 40 degrees past center without dispenser. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. 005 OD Normal and Rapid modes, 96- and 384-well plates Fluorescence Read Timing Excitation Filter: 485/20 nm Emission Filter: 528/20 nm 96-well read: 89 sec. Get Quote . Mix samples in microplate wells by user-programmable Automix feature. Detection cartridge modularity allows for easy addition of new read modes within minutes without. 5 fg per well to 0. Selects either 96-well or 384-well plate for a stand-alone read. The SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . ©2022 Molecular Devices - All rights. Dual injectors for kinetic assays. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. the use of a reader calibrated with primary NIST and NMI standards. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. For the kinetic and endpoint LAL assays, the ELx808 TM Reader is highly reliable and easy to use. Specifications subject to change without notice. #ABS Plus) Cell plating and treatment prior to assay. Cayman Chemical. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). A standard 96 well plate was set up as described in Protocol Table 4. Validation made easy -1) Select the correct plate and the wells that you want to measure. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data analysis with SoftMax® Pro Software Optimal performance with validation tools Small but Mighty EarlyTox Live/Dead Assay Kit (Molecular Devices cat. Intuitive graphical user interface makes each assay extremely easy to set. . luminescence for a 96-well plate and 40 msec integration time for a 384-well plate: 0517 Lit.